Flow cytometry analysis on BM: 78% blasts with intermediate to high side scatter, coexpression of CD13 (variable), CD33, CD117, CD34 (partial), HLA-DR, cMPO, CD4(dim), no expression of CD14, CD11b, CD56, T-cell or B-cell markers.
Karyotype: 46 XY.
FISH: negative for AML panel including RARA, RUNX1, CBFB-MYH11, MLL, RPN1-EV1, RBM15-MKL1
Molecular tests: negative for mutations in NPM1, CEBPA, FLT3, PML-RARA
Diagnosis: AML with maturation (WHO 2008), FAB, M2
Karyotype: 46 XY.
FISH: negative for AML panel including RARA, RUNX1, CBFB-MYH11, MLL, RPN1-EV1, RBM15-MKL1
Molecular tests: negative for mutations in NPM1, CEBPA, FLT3, PML-RARA
Diagnosis: AML with maturation (WHO 2008), FAB, M2