Flow cytometry study:
A CD45+ blast population is present with low side scatter. Positive: CD34, CD38, CD117, CD33, CD11b, CD56, CD7, CD71; partially positive: CD61, CD13, CD4, HLA-dr, CD123; negative: CD14, CD15, MPO, CD235a, Lysozyme, TdT, B-cell and other T-cell markers.
Diagnosis: Myeloid proliferation related to Down syndrome: transient abnormal myelopoiesis VS myeloid leukemia associated with Down syndrome
The two are similar in morphology and immunophenotype, so distinction can't be made based on morphology and/or immunophenotype alone. TAM usually remits within few weeks without treatment. So clinical course can definitely make the call. Additional cytogenetic abnormalities and/or molecular other than GATA1 mutation favor myeloid leukemia.
A CD45+ blast population is present with low side scatter. Positive: CD34, CD38, CD117, CD33, CD11b, CD56, CD7, CD71; partially positive: CD61, CD13, CD4, HLA-dr, CD123; negative: CD14, CD15, MPO, CD235a, Lysozyme, TdT, B-cell and other T-cell markers.
Diagnosis: Myeloid proliferation related to Down syndrome: transient abnormal myelopoiesis VS myeloid leukemia associated with Down syndrome
The two are similar in morphology and immunophenotype, so distinction can't be made based on morphology and/or immunophenotype alone. TAM usually remits within few weeks without treatment. So clinical course can definitely make the call. Additional cytogenetic abnormalities and/or molecular other than GATA1 mutation favor myeloid leukemia.