Flow cytometry of the PB:
Cytogenetic studies: 47,XY,dup(5)(q14q32),+21[12]; 48,sl,+10[2]; 46,XY[6]
Microarray:
Microarray:
Microarray analysis shows: 1. Gain of the 5q14.1q32 region with breaks at the SSBP2 and CSF1R genes. This interstitial duplication likely results in SSBP2/CSF1R fusion. 2. IKZF1 deletion exons1-6.
Multiplex RT-PCR gene fusion test: positive for kinase gene fusion SSBP2/CSF1R.
Diagnosis: B-cell acute lymphoblastic leukemia, Philadelphia-like (B-ALL, Ph-like.
Multiplex RT-PCR gene fusion test: positive for kinase gene fusion SSBP2/CSF1R.
Diagnosis: B-cell acute lymphoblastic leukemia, Philadelphia-like (B-ALL, Ph-like.